Analysis Note

Controlhuman lymphocytes

Application

This Milli-Mark Anti-CD5-FITC Antibody, clone DK23 is validated for use in FC for the detection of CD5.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD5 is a transmembrane glycoprotein monomer with an Mr of 67,000. It possesses a large cytoplasmic domain suitable for signal transduction (Lozano, 1997). CD5 appears early in thymocyte development (Lozano, 1997), and is expressed at low density on thymocytes and at high density on all mature T lymphocytes (Lozano, 1997). The specificity of anti-CD5, DK23, is equivalent to that of the CD5-clustered antibody Leu-1 (Stein, 1984).

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Quality

Evaluated by flow cytometry using human lymphocytes

Specificity

Antibody recognizes Human CD5 antigen.

Storage and Stability

Maintain refrigerated at 2-8°C, protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

67 kDa (Lozano, 1997)